Culture and purification of mononuclear cells from peripheral blood and lymphoid tissue.

Selective isolation of lymphocyte subpopulations (T-reg-Th17-TCD4-TCD8-B-DC-dc cells).

Generation of dendritic cells from monocytes.

Generation of monoclonal antibodies from hybridomas.

Analysis of lymphocyte subpopulations.

Phenotypic determination and functional analysis of B and T lymphocytes, monocytes, dendritic cells and granulocytes with extra and intracytoplasmic staining.

SE acetate lymphoproliferation with carboxy-fluorescein.

Measurement of intracellular nitroxide (NO) (DAF –FM).

Studies on phagocytosis.

Use of pentamers (MHC-peptide) for the recognition of peptide-specific T lymphocytes.

Chemokines, cytokines, perforins and granzymes and transcription factors and dosage of their receptors.

Stimulation with mitogens and determination of proliferative activity.

IFN-γ, TNF-α IL-2 and IL-6. Biological dosage.

Dosage of nitroxide (NO) in biological fluids or tissue homogenate.

Myeloperoxidase (MPO) assay in biological fluids or tissue homogenate.

Dosage by ELISA and RIA techniques of different analytes (e.g. Cytokines, hormones, proteins, antibodies).

Detection of antibodies by immunofluorescence techniques.

Production and purification of monoclonal antibodies.

In vitro test for chemotaxis.